BACKGROUND Leptin, as a 16 kDa adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. It also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. The aim of this study was to investigate the effects of in vitro addition of leptin t...

the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...

To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes, and 14...

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative quantitative features of native buffalo ovaries oocytes to understand them comprehensively. addition, it was conducted pick the ideal for oocyte collection assess either maturation affects diameter or not. The were collected from local slaughterhouses Dhaka city...

Background: There is a considerable numbers of buffalo (Bubalus bubalis) in Iran which have an important role in rural life. The reproductive performance of buffalo is lower than cow. Co-culture with somatice cells improved the maturation rate of most of the species. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on post fertilization development of buffalo...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

Interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. In an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (Bos taurus) and water buffalo (Bubalus bubalis), cattle oocytes were e...

Background: The buffalo (Bubalus bubalis) is the animal with special production characteristics in producing milk and meat has a critical role in rural industry in Iran. The most problem of this specie is its low reproductive output. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on maturation rate of Buffalo oocyte.Materials and Methods: Ovaries were colle...

Leptin, the 16-kDa product of the obese (ob) gene, primarily secreted from adipose tissue, has been implicated to play an important role in the regulation of food intake and energy expenditure. This study investigated protective effect of leptin on trichostatin A-induced apoptotic on in vitro maturation ratio of buffalo oocytes. Ovaries were collected from abattoir and were transported...

Follicular fluid (FF) accumulates in the antrum of the ovarian follicle and provides the microenvironment for oocyte development. FF plays an important role in follicle growth and oocyte maturation. The FF provides a unique window to investigate the processes occurring during buffalo follicular development. The observed low quality of buffalo oocytes may arise from the poor follicular microenvi...